4WM1

High pressure protein crystallography of hen egg white lysozyme at 500 MPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	298
Detector technology	CCD
Collection date	2013-10-30
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.75
Spacegroup name	P 43 21 2
Unit cell lengths	77.287, 77.287, 37.938
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.340 - 1.600
R-factor	0.16246
Rwork	0.160
R-free	0.20795
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.025
RMSD bond angle	2.157
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	15744
<I/σ(I)>	46.2
Completeness [%]	97.4
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	Sodium chloride