4WLY

High pressure protein crystallography of hen egg white lysozyme at 380 MPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	298
Detector technology	CCD
Collection date	2013-01-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.71
Spacegroup name	P 43 21 2
Unit cell lengths	77.510, 77.510, 37.967
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.420 - 1.620
R-factor	0.15646
Rwork	0.155
R-free	0.19376
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	2.099
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.620
Number of reflections	15293
<I/σ(I)>	50.3
Completeness [%]	92.7
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	Sodium chloride