4WLV

Crystal structure of NAD bound MDH2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 21 21 21
Unit cell lengths	59.864, 152.016, 155.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.860 - 2.400
R-factor	0.21057
Rwork	0.209
R-free	0.24787
RMSD bond length	0.005
RMSD bond angle	1.182
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.860
High resolution limit [Å]	2.400
Number of reflections	53618
<I/σ(I)>	51.2
Completeness [%]	99.9
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	288	0.1 M Na2HPO4:citric acid, 40% (v/v) polyethylene glycol 300, 10% DMSO