4WLQ

Crystal structure of mUCH37-hRPN13 CTD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-09-02
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	59.935, 96.843, 98.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.144 - 2.850
R-factor	0.1996
Rwork	0.192
R-free	0.27800
RMSD bond length	0.009
RMSD bond angle	1.212
Data reduction software	XDS
Data scaling software	Aimless

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.260	38.260	3.000
High resolution limit [Å]	2.850	9.010	2.850
Rmerge	0.112	0.077	1.003
Rpim	0.042	0.031	0.375
Total number of observations	110961	3504	15299
Number of reflections	14095
<I/σ(I)>	12.2	26.5	2.4
Completeness [%]	100.0	99	100
Redundancy	7.9	6.8	7.6
CC(1/2)	0.997	0.996	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	277	20% PEG 3350, 200 mM Mg(OAC)2,100 mM HEPES