4WLN

Crystal structure of apo MDH2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 21 21 21
Unit cell lengths	59.919, 151.271, 155.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.560 - 2.280
R-factor	0.21828
Rwork	0.217
R-free	0.24621
RMSD bond length	0.005
RMSD bond angle	1.226
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.560
High resolution limit [Å]	2.280
Number of reflections	62209
<I/σ(I)>	34.04
Completeness [%]	99.8
Redundancy	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	288	0.1 M Na2HPO4:citric acid, 40% (v/v) polyethylene glycol 300, 10% DMSO