4WJP
Crystal Structure of SUMO1 in complex with phosphorylated Daxx
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.266, 38.069, 73.950 |
| Unit cell angles | 90.00, 101.04, 90.00 |
Refinement procedure
| Resolution | 33.713 - 1.700 |
| R-factor | 0.1645 |
| Rwork | 0.163 |
| R-free | 0.19520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uyz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.253 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_1555)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.042 | 0.412 |
| Number of reflections | 20835 | |
| <I/σ(I)> | 15.3 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM sodium cacodylate, 26% PEG3350, 10mM CaCl2 |
