4WJN
Crystal structure of SUMO1 in complex with phosphorylated PML
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-14 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.274, 47.079, 63.986 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.921 - 1.500 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.19210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uyz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.298 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_1555)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.921 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.112 | 0.870 |
| Number of reflections | 18507 | |
| <I/σ(I)> | 11.5 | 2.1 |
| Completeness [%] | 96.7 | 81.8 |
| Redundancy | 9.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM sodium cacodylate, 16% PEG3350, 10mM calcium chloride |
