4WJ8
Human Pyruvate Kinase M2 Mutant C424A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.359, 115.260, 109.401 |
| Unit cell angles | 90.00, 114.43, 90.00 |
Refinement procedure
| Resolution | 49.880 - 2.870 |
| R-factor | 0.2024 |
| Rwork | 0.200 |
| R-free | 0.24318 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3srd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.069 |
| Phasing software | MOLREP (11.2.05) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.880 |
| High resolution limit [Å] | 2.870 |
| Rmerge | 0.094 |
| Number of reflections | 43988 |
| <I/σ(I)> | 8.3 |
| Completeness [%] | 90.5 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 3350, sodium cacodylate, Triethanolamine, MgCl2, KCl |
