4WIB

Crystal structure of Magnesium transporter MgtE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-27
Detector	RAYONIX MX225HE
Wavelength(s)	0.97914
Spacegroup name	P 21 21 21
Unit cell lengths	64.120, 70.380, 103.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.083 - 3.200
R-factor	0.2419
Rwork	0.240
R-free	0.26770
RMSD bond length	0.004
RMSD bond angle	1.011
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200
Number of reflections	7850
<I/σ(I)>	1.67
Completeness [%]	90.6
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	PEG400, CaCl2, NaSCN, HEPES