4WI9

Structural mapping of the human IgG1 binding site for FcRn: hu3S193 Fc mutation I253A/H310A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-13
Detector	ADSC QUANTUM 210
Wavelength(s)	0.954
Spacegroup name	P 21 21 21
Unit cell lengths	49.163, 77.654, 140.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.782 - 2.650
R-factor	0.2131
Rwork	0.206
R-free	0.27370
RMSD bond length	0.008
RMSD bond angle	1.272
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.650
Number of reflections	16296
<I/σ(I)>	24.5
Completeness [%]	99.5
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	PEG 6000, 0.1 M MES, 25% v/v ethylene glycol