4WI8

Structural mapping of the human IgG1 binding site for FcRn: hu3S193 Fc mutation Y436A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-04
Detector	ADSC QUANTUM 210
Wavelength(s)	0.954
Spacegroup name	P 21 21 21
Unit cell lengths	49.109, 79.298, 136.341
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.877 - 2.800
R-factor	0.2177
Rwork	0.209
R-free	0.29360
RMSD bond length	0.009
RMSD bond angle	1.415
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Number of reflections	13774
<I/σ(I)>	22.6
Completeness [%]	97.7
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	PEG 6000, 0.1 M MES, 25% v/v ethylene glycol