4WHT
Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody 3/11, P1 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97902 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.794, 128.225, 163.626 |
| Unit cell angles | 88.79, 94.36, 96.15 |
Refinement procedure
| Resolution | 32.500 - 2.220 |
| R-factor | 0.2108 |
| Rwork | 0.209 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.740 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.383 | |
| Number of reflections | 246066 | |
| <I/σ(I)> | 9 | 1.8 |
| Completeness [%] | 95.4 | 81.4 |
| Redundancy | 2.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM TRIS, 27% PEG4000, 100mM Na-Acetate |
