4WH4

Protein GB1 Quadruple Mutant I6H/N8H/K28H/Q32H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-05
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	74.287, 74.287, 41.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.167 - 2.200
R-factor	0.2068
Rwork	0.201
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qmt
RMSD bond length	0.004
RMSD bond angle	0.647
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall
Low resolution limit [Å]	32.170
High resolution limit [Å]	2.200
Rmerge	0.070
Number of reflections	6876
<I/σ(I)>	14
Completeness [%]	99.7
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.75 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M HEPES pH 7.5