4WH2
N-acetylhexosamine 1-kinase in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-15 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.079, 88.220, 105.895 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.399 - 1.847 |
R-factor | 0.1968 |
Rwork | 0.194 |
R-free | 0.24200 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.016 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.847 | 1.850 |
Rmerge | 0.080 | 0.507 |
Number of reflections | 36058 | |
<I/σ(I)> | 27.8 | 3.7 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG8000, magnesium acetate, sodium cacodylate |