4WG1
Room temperature crystal structure of lysozyme determined by serial synchrotron crystallography (micro focused beam - crystFEL)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2014-04-19 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.954 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.940, 77.940, 38.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.112 - 1.700 |
R-factor | 0.2277 |
Rwork | 0.226 |
R-free | 0.25460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jln |
RMSD bond length | 0.006 |
RMSD bond angle | 0.864 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.100 | 1.780 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 13496 | |
<I/σ(I)> | 3.4 | 0.64 |
Completeness [%] | 100.0 | 100 |
Redundancy | 114.8 | 18.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 50mM Sodium acetate pH 4.5, 16% PEG 4K, 3.5M NaCl |