4WFZ

Coxsackievirus B3 3Dpol RNA Dependent RNA Polymerase - NaCl Crystal Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-09-29
Detector	NOIR-1
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	74.423, 74.423, 288.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.876 - 1.803
R-factor	0.2008
Rwork	0.200
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ddk
RMSD bond length	0.008
RMSD bond angle	1.139
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	58.900
High resolution limit [Å]	1.800
Number of reflections	75393
<I/σ(I)>	12.81
Completeness [%]	98.2
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	289	0.085 M HEPES, 3.6 M NaCl , 15% Glycerol