4WFW
The crystal structure of Dickeya dadantii GspB from the type 2 secretion system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 36.210, 36.210, 131.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.910 - 2.050 |
| R-factor | 0.21303 |
| Rwork | 0.209 |
| R-free | 0.25421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oss |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.682 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0085) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.030 |
| High resolution limit [Å] | 2.020 |
| Number of reflections | 6380 |
| <I/σ(I)> | 30.6 |
| Completeness [%] | 99.2 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | initial hit from nano drop. Scaled up using whisker streak seeding. 0.2M MgCl, 0.1M Tris, 30% w/v PEG4000, pH8.5 |
