4WFL
Structure of the complete bacterial SRP Alu domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.60549, 1.60489, 1.03321 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.466, 68.283, 82.991 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.495 - 2.490 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.20900 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.587 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.090 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 12953 | |
| <I/σ(I)> | 12 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.5 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | sodium cacodylate, ammonium acetate, magnesium acetate, PEG8000, jeffamine-M600 |
