4WFD

Structure of the Rrp6-Rrp47-Mtr4 interaction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-22
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	P 3 1 2
Unit cell lengths	142.745, 142.745, 63.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.420 - 2.400
R-factor	0.2028
Rwork	0.201
R-free	0.24260
Structure solution method	SAD
Starting model (for MR)	4wfc
RMSD bond length	0.009
RMSD bond angle	1.290
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.420		2.490
High resolution limit [Å]	2.400	7.120	2.400
Rmerge	0.094	0.017
Rmeas	0.095	0.018	1.784
Total number of observations	500154
Number of reflections	56538	2160	8939
<I/σ(I)>	25.7	99.66	1.7
Completeness [%]	99.5	99.5	96.5
Redundancy	17.3		17.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	12% PEG 1000, 0.1M imidazole pH 7.5, 0.125 M calcium acetate, 5mM yttrium chloride