4WF9

The crystal structure of the large ribosomal subunit of Staphylococcus aureus in complex with telithromycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.973
Spacegroup name	P 65 2 2
Unit cell lengths	282.660, 282.660, 877.075
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.188 - 3.427
R-factor	0.1939
Rwork	0.192
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wce
RMSD bond length	0.006
RMSD bond angle	1.106
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.460
High resolution limit [Å]	3.400	9.210	3.400
Rmerge	0.165	0.096
Rmeas	0.177	0.104
Rpim	0.063	0.036	0.492
Total number of observations	1836944
Number of reflections	268208
<I/σ(I)>	5.7
Completeness [%]	98.3	96.9	94.4
Redundancy	6.8	7.4	4.4
CC(1/2)		0.991	0.473

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Hepes, KCl, MgCl2, NH4Cl, MPD, EtOH, Spermidine