4WF1

Crystal structure of the E. coli ribosome bound to negamycin.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	211.910, 434.310, 624.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.136 - 3.090
R-factor	0.2019
Rwork	0.202
R-free	0.24440
RMSD bond length	0.008
RMSD bond angle	1.252
Data scaling software	Aimless (0.2.17)
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.140	41.140	3.270
High resolution limit [Å]	3.090	9.260	3.090
Rmerge	0.130	0.028	0.804
Rpim	0.058	0.013	0.358
Total number of observations	6209734	222208	1008425
Number of reflections	1043787
<I/σ(I)>	14.9	61.3	2.6
Completeness [%]	99.9	98.7	100
Redundancy	5.9	5.6	6
CC(1/2)	0.994	0.994	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	PEG8k, MPD