4WEN
Co-complex structure of the F4 fimbrial adhesin FaeG variant ac with llama single domain antibody V2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 145.833, 145.833, 37.875 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.740 - 1.890 |
| R-factor | 0.19016 |
| Rwork | 0.188 |
| R-free | 0.22599 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hlr |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.106 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.740 | 2.000 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 36784 | |
| <I/σ(I)> | 14.3 | 1.4 |
| Completeness [%] | 99.7 | 97.7 |
| Redundancy | 20.2 | 19.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 100 mM Sodium Acetate pH 4.6, 200 mM ammonium sulfate, 25 % w/v PEG 4000 |
