4WEE
High-resolution structure of Synaptotagmin 1 C2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Detector technology | CCD |
| Collection date | 2008-01-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.81798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.738, 38.547, 44.295 |
| Unit cell angles | 90.00, 97.94, 90.00 |
Refinement procedure
| Resolution | 19.781 - 0.891 |
| R-factor | 0.1502 |
| Rwork | 0.149 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rsy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.550 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.030 |
| High resolution limit [Å] | 0.700 | 0.890 |
| Rmerge | 0.020 | |
| Number of reflections | 97100 | |
| <I/σ(I)> | 50 | 2 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 1.5M ammonium sulfate, 1% PEG400 |
