4WEA

Structure and receptor binding prefereneces of recombinant human A(H3N2) virus hemagglutinins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-04-20
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	100.733, 100.733, 382.613
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.039 - 2.200
R-factor	0.1912
Rwork	0.190
R-free	0.22300
RMSD bond length	0.006
RMSD bond angle	1.021
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	38431
<I/σ(I)>	7.1
Completeness [%]	99.6
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	10.5	293	0.1M CAPS:NaOH, 30% PEG 400