4WE5

The crystal structure of hemagglutinin from A/Port Chalmers/1/1973 influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-23
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.0
Spacegroup name	I 21 3
Unit cell lengths	153.128, 153.128, 153.128
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.031 - 2.100
R-factor	0.1842
Rwork	0.182
R-free	0.23070
RMSD bond length	0.007
RMSD bond angle	1.041
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	34697
<I/σ(I)>	27.3
Completeness [%]	99.5
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	293	0.1M HEPES, 25% PEG 550MME