4WD0
Crystal structure of HisAp form Arthrobacter aurescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 6 |
| Unit cell lengths | 93.974, 93.974, 45.433 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.470 - 1.500 |
| R-factor | 0.1373 |
| Rwork | 0.135 |
| R-free | 0.17319 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1axk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.574 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.069 | 0.773 |
| Number of reflections | 36750 | |
| <I/σ(I)> | 19 | 1.78 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.8 M LiCl, 0.1 M Tris/HCl, pH 8.5, 32% PEG 4000, 2 mM RCDRP, cryo 5% glycerol |
