4WCT
The crystal structure of Fructosyl amine: oxygen oxidoreductase (Amadoriase I) from Aspergillus fumigatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.396, 83.100, 176.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.070 - 1.670 |
| R-factor | 0.14083 |
| Rwork | 0.139 |
| R-free | 0.16866 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3djd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.345 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.070 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.473 | |
| Number of reflections | 119826 | |
| <I/σ(I)> | 28.94 | 3.4 |
| Completeness [%] | 98.8 | 95 |
| Redundancy | 5.6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Sodium citrate 0.1M, 10% PEG 4000, 15% 2-propanol |
