4WCK

Crystal Structure of apo Cell Shape Determinant protein Csd4 from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	53.280, 66.780, 145.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.990 - 1.400
R-factor	0.143
Rwork	0.142
R-free	0.15900
RMSD bond length	0.013
RMSD bond angle	1.486
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.990	1.440
High resolution limit [Å]	1.400	1.400
Rmerge	0.064	0.598
Number of reflections	197473
<I/σ(I)>	12.56	2.39
Completeness [%]	99.9	99.6
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	16-20% PEG 3350, 0-0.1 mM Tris, and 0.3-0.4 M NaI