4WCE

The crystal structure of the large ribosomal subunit of Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873, 1.000
Spacegroup name	P 65 2 2
Unit cell lengths	279.756, 279.756, 872.725
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.736 - 3.526
R-factor	0.204
Rwork	0.202
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zjr
RMSD bond length	0.006
RMSD bond angle	1.161
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.570
High resolution limit [Å]	3.510	9.510	3.510
Rmerge	0.253	0.140	0.978
Rmeas	0.256	0.148
Rpim	0.066	0.043	0.343
Total number of observations	2830709
Number of reflections	236855
<I/σ(I)>	4.8
Completeness [%]	96.3	94.1	84.3
Redundancy	12	9.7	6
CC(1/2)		0.979	0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Hepes, KCl, MgCl2, NH4Cl. MPD, EtOH, Spermidine