4WCE
The crystal structure of the large ribosomal subunit of Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873, 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 279.756, 279.756, 872.725 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.736 - 3.526 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zjr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.161 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.570 |
| High resolution limit [Å] | 3.510 | 9.510 | 3.510 |
| Rmerge | 0.253 | 0.140 | 0.978 |
| Rmeas | 0.256 | 0.148 | |
| Rpim | 0.066 | 0.043 | 0.343 |
| Total number of observations | 2830709 | ||
| Number of reflections | 236855 | ||
| <I/σ(I)> | 4.8 | ||
| Completeness [%] | 96.3 | 94.1 | 84.3 |
| Redundancy | 12 | 9.7 | 6 |
| CC(1/2) | 0.979 | 0.633 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Hepes, KCl, MgCl2, NH4Cl. MPD, EtOH, Spermidine |
