4WBX

Conserved hypothetical protein PF1771 from Pyrococcus furiosus solved by sulfur SAD using Swiss Light Source data

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-30
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	2.066
Spacegroup name	F 2 2 2
Unit cell lengths	91.020, 126.820, 155.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.627 - 2.301
R-factor	0.2378
Rwork	0.233
R-free	0.27720
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.265
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.301
Rmerge	0.063
Number of reflections	18037
<I/σ(I)>	38.9
Completeness [%]	99.3
Redundancy	62.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	0.07 M SODIUM ACETATE BUFFER CONTAINING 0.14 M CACL2 14% (V/V) ISOPROPANOL AND 30% (V/V) GLYCEROL