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4WBL

Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, with mutation F235A

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron sitePETRA III, EMBL c/o DESY
BeamlineP14 (MX2)
Temperature [K]100
Detector technologyCCD
Collection date2012-05-19
DetectorRAYONIX MX-225
Wavelength(s)1.223
Spacegroup nameP 1 21 1
Unit cell lengths40.220, 47.770, 54.090
Unit cell angles90.00, 94.73, 90.00
Refinement procedure
Resolution27.437 - 2.501
R-factor0.1983
Rwork0.195
R-free0.26490
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2xmi
RMSD bond length0.002
RMSD bond angle0.606
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX ((phenix.refine: 1.9_1692))
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]30.0002.570
High resolution limit [Å]2.50011.1902.500
Rmerge0.1520.0640.769
Rmeas0.1780.0790.904
Total number of observations25813
Number of reflections716784515
<I/σ(I)>6.8515.311.85
Completeness [%]99.790.3100
Redundancy3.63.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP298Na-acetate, PEG 4000/6000

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