4WB4

wt SA11 NSP4_CCD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9999
Spacegroup name	I 2 2 2
Unit cell lengths	31.970, 35.720, 178.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.710 - 2.030
R-factor	0.2361
Rwork	0.234
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o1k
RMSD bond length	0.008
RMSD bond angle	0.930
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	44.710
High resolution limit [Å]	2.030
Rmerge	0.030
Number of reflections	6998
<I/σ(I)>	13.4
Completeness [%]	99.3
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	296	25% w/v PEG 1500, 0.1M MIB buffer (pH 7.0)