4WAU
Crystal structure of CENP-M solved by native-SAD phasing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-10 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 2.0664 |
| Spacegroup name | P 3 |
| Unit cell lengths | 104.570, 104.570, 33.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.280 - 2.200 |
| R-factor | 0.1252 |
| Rwork | 0.117 |
| R-free | 0.14760 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.551 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.139 | |
| Number of reflections | 40969 | |
| <I/σ(I)> | 92.37 | 16.94 |
| Completeness [%] | 98.1 | 86.8 |
| Redundancy | 140.4 | 32.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 100 mM bicine pH 8.5, 11 % MPD, 8 mM spermidine |
