4WAU

Crystal structure of CENP-M solved by native-SAD phasing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-10
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	2.0664
Spacegroup name	P 3
Unit cell lengths	104.570, 104.570, 33.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.280 - 2.200
R-factor	0.1252
Rwork	0.117
R-free	0.14760
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.551
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge		0.139
Number of reflections	40969
<I/σ(I)>	92.37	16.94
Completeness [%]	98.1	86.8
Redundancy	140.4	32.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	100 mM bicine pH 8.5, 11 % MPD, 8 mM spermidine