4WAM
H. influenzae beta-carbonic anhydrase variant W39V/G41A/P48S/A49P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-05-28 |
| Detector | OXFORD RUBY CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 48.162, 130.259, 143.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.560 - 2.200 |
| R-factor | 0.23508 |
| Rwork | 0.232 |
| R-free | 0.28681 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.793 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.560 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.521 | |
| Number of reflections | 22403 | |
| <I/σ(I)> | 7.2 | 1.7 |
| Completeness [%] | 95.3 | 83.1 |
| Redundancy | 3.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.30 M sodium potassium phosphate, 12 mg/mL protein |
