4WAL

Crystal structure of selenomethionine Msl5 protein in complex with RNA at 2.2 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-22
Detector	PSI PILATUS 6M
Wavelength(s)	0.97919
Spacegroup name	P 4 2 2
Unit cell lengths	81.952, 81.952, 51.495
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.650 - 2.200
R-factor	0.174
Rwork	0.172
R-free	0.21500
RMSD bond length	0.007
RMSD bond angle	1.050
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.650	2.300
High resolution limit [Å]	2.180	2.180
Rmerge	0.108
Number of reflections	9666
<I/σ(I)>	40.2
Completeness [%]	100.0	100
Redundancy	39.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	295	2.1-2.2M ammonium sulfate, 0.01M Mg Acetate, 0.05M MES