4W9T

Crystal structure of HisAP from Streptomyces sp. Mg1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	69.332, 69.332, 126.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.859 - 1.570
R-factor	0.1553
Rwork	0.154
R-free	0.18350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vzw
RMSD bond length	0.011
RMSD bond angle	1.442
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.600
High resolution limit [Å]	1.570	1.570
Rmerge	0.066	1.000
Number of reflections	43584
<I/σ(I)>	34.9	2.1
Completeness [%]	99.8	98
Redundancy	11.7	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	2 M (NH4)2SO4, 0.1 M Bis/Tris, protein 10 mg/ml, pH 5.5