4W8Z
Crystal structure of Cmr1 from Pyrococcus furiosus (apo form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.037 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 100.757, 100.757, 106.932 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.589 - 2.700 |
| R-factor | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.21950 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.690 |
| Data reduction software | XDS |
| Refinement software | PHENIX ((phenix.refine: 1.9_1678)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.798 |
| High resolution limit [Å] | 2.700 | 2.701 |
| Number of reflections | 33393 | |
| <I/σ(I)> | 18.2 | 1.3 |
| Completeness [%] | 99.8 | 97.88 |
| Redundancy | 19.4 | 17.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 100 mM bicine/Trizma base pH 6.0, 10% PEG 8000, 20% ethylene glycol, 20 mM each of sodium DL- alanine, sodium L-gutamate, gylcine, DL-lysine HCl, DL-serine and either 3% DMSO or 3% D-sorbitol |
