4W8O

Structure of the luciferase-like enzyme from the nonluminescent Zophobas morio mealworm

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.456
Spacegroup name	P 21 21 21
Unit cell lengths	87.774, 91.005, 110.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.110 - 2.050
R-factor	0.1899
Rwork	0.188
R-free	0.23190
RMSD bond length	0.006
RMSD bond angle	1.033
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.110
High resolution limit [Å]	2.050
Number of reflections	53794
<I/σ(I)>	24.06
Completeness [%]	95.7
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	100 mM HEPES, 200 mM ammonium chloride, 16% (w/v) PEG 6000