4W8L
Structure of GH10 from Paenibacillus barcinonensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 32 |
| Unit cell lengths | 126.689, 126.689, 57.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.570 - 1.760 |
| R-factor | 0.21956 |
| Rwork | 0.218 |
| R-free | 0.25153 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q8x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.147 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.570 | 1.860 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.106 | 0.494 |
| Number of reflections | 102672 | |
| <I/σ(I)> | 5.1 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 23% PEG 3350, 0.1 M potassium thiocyanate, 0.1 M sodium acetate |
