4W7Z
Tetrameric BAP29 vDED without disulfide bonds
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 115.900, 115.900, 167.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.265 - 2.200 |
| R-factor | 0.1842 |
| Rwork | 0.183 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model at lower resolution build based on selenomethionine SAD phases |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.976 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.270 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 28934 | |
| <I/σ(I)> | 19.05 | |
| Completeness [%] | 98.9 | 97.1 |
| Redundancy | 10.1 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 277.15 | The crystal grew in 1.8 M ammonium sulfate, 100 mM Na acetate pH 4.6 and 5 mM TCEP using protein purified in 20 mM Tris pH 8, 150 mM NaCl and 0.5 mM TCEP and concentrated to ~14 mg/mL |
