4W7X
Crystal Structure of Full-Length Split GFP Mutant E115C Disulfide Dimer, P 1 21 1 Space Group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.840, 70.470, 116.780 |
| Unit cell angles | 90.00, 92.56, 90.00 |
Refinement procedure
| Resolution | 66.770 - 2.800 |
| R-factor | 0.223 |
| Rwork | 0.217 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.864 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1555)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 66.770 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 26887 |
| <I/σ(I)> | 8.19 |
| Completeness [%] | 99.7 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 1.0 M (NH4)2HPO4, acetate pH 4.5 |
