4W7R

Crystal Structure of Full-Length Split GFP Mutant E124H/K126H Copper Mediated Dimer, P 21 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9789
Spacegroup name	P 1 21 1
Unit cell lengths	62.670, 87.190, 92.070
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	92.070 - 1.800
R-factor	0.226
Rwork	0.223
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.015
RMSD bond angle	1.382
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	92.070
High resolution limit [Å]	1.799
Number of reflections	90790
<I/σ(I)>	6.36
Completeness [%]	98.8
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG3350, 0.2M Potassium formate