4W7K
CRYSTAL STRUCTURE OF A DECOLORIZING PEROXIDASE (DYP) FROM AURICULARIA AURICULA-JUDAE. Y147S MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.950, 55.870, 104.000 |
| Unit cell angles | 90.00, 118.26, 90.00 |
Refinement procedure
| Resolution | 33.627 - 1.050 |
| R-factor | 0.1364 |
| Rwork | 0.135 |
| R-free | 0.15680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4afv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.410 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.110 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 1.052 | |
| Number of reflections | 364516 | |
| <I/σ(I)> | 12.3 | 1 |
| Completeness [%] | 82.6 | 29.8 |
| Redundancy | 6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 35% PEG 2000 MME |
