4W72

Crystal Structure of Full-Length Split GFP Mutant E115C/T118H Disulfide Dimer With Copper Mediated Crystal Contacts, P 21 21 21, Form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	72.540, 74.400, 89.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.120 - 2.000
R-factor	0.194
Rwork	0.190
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.008
RMSD bond angle	1.189
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	57.120
High resolution limit [Å]	1.996
Number of reflections	33399
<I/σ(I)>	16.59
Completeness [%]	99.7
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	20% PEG3000, 0.1M Acetate pH 4.5