4W6Y
Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.562, 102.961, 114.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.260 - 1.570 |
| R-factor | 0.19648 |
| Rwork | 0.195 |
| R-free | 0.22567 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4B4P and 2X1O |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.968 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.950 | 1.660 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 1.837 | |
| Number of reflections | 42810 | |
| <I/σ(I)> | 11.1 | 1 |
| Completeness [%] | 98.6 | 95.5 |
| Redundancy | 9.7 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2 M (NH4)2 SO4, 5% PEG-400 and 100 mM MES pH 6.5 |
