4W6X
Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.310, 78.610, 108.935 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.750 - 1.880 |
| R-factor | 0.18759 |
| Rwork | 0.186 |
| R-free | 0.22601 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x1o |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.994 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.770 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.951 | |
| Number of reflections | 21548 | |
| <I/σ(I)> | 11.2 | 2.1 |
| Completeness [%] | 97.4 | 85.6 |
| Redundancy | 5.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 1 M (NH4)2 SO4, 90 mM Na-citrate pH 4.0 |
