4W6U

Crystal Structure of Full-Length Split GFP Mutant E115H/T118H With Nickel Mediated Crystal Contacts, P 21 21 21 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	47.640, 116.580, 165.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.990 - 2.280
R-factor	0.214
Rwork	0.210
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.010
RMSD bond angle	1.433
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	82.990
High resolution limit [Å]	2.278
Number of reflections	42930
<I/σ(I)>	14.2
Completeness [%]	99.3
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	0.2M NaCl, 0.1M Phosphate-citrate pH 4.2, 20% PEG8000