4W6U
Crystal Structure of Full-Length Split GFP Mutant E115H/T118H With Nickel Mediated Crystal Contacts, P 21 21 21 Space Group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.640, 116.580, 165.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 82.990 - 2.280 |
R-factor | 0.214 |
Rwork | 0.210 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.433 |
Refinement software | PHENIX ((PHENIX.REFINE: DEV_1555)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 82.990 |
High resolution limit [Å] | 2.278 |
Number of reflections | 42930 |
<I/σ(I)> | 14.2 |
Completeness [%] | 99.3 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 0.2M NaCl, 0.1M Phosphate-citrate pH 4.2, 20% PEG8000 |