4W6T

Crystal Structure of Full-Length Split GFP Mutant E115H/T118H With Copper Mediated Crystal Contacts, P 43 21 2 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	105.300, 105.300, 69.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.460 - 1.604
R-factor	0.182
Rwork	0.180
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.019
RMSD bond angle	1.758
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	74.460
High resolution limit [Å]	1.604
Number of reflections	51580
<I/σ(I)>	17.81
Completeness [%]	99.8
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.15M Kbr, 30% PEG MME 2000