4W6S
Crystal Structure of Full-Length Split GFP Mutant K126C Disulfide Dimer, P 43 21 2 Space Group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 91.510, 91.510, 205.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.280 - 3.100 |
| R-factor | 0.2285 |
| Rwork | 0.223 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.550 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.280 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 16549 |
| <I/σ(I)> | 18.03 |
| Completeness [%] | 100.0 |
| Redundancy | 25.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 40% PEG300, 0.1M Phosphate-citrate pH 4.2 |
