4W6R

Crystal Structure of Full-Length Split GFP Mutant D102C Disulfide Dimer, P 1 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	92.420, 92.560, 124.530
Unit cell angles	94.94, 96.17, 102.25

Refinement procedure

Resolution	89.880 - 3.471
R-factor	0.312
Rwork	0.307
R-free	0.35700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.010
RMSD bond angle	1.841
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	89.880
High resolution limit [Å]	3.471
Number of reflections	46082
<I/σ(I)>	5.7
Completeness [%]	89.4
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG3350, 0.2M NaSCN